Title: GAD1 Gene Expression in Blood of Patients with First-Episode Psychosis
year: 2017
Journal: PLoS One
Volume: 12
Issue: 1
Pages: e0170805
ISSN: 1932-6203 (Electronic);1932-6203 (Linking)
Legal note: PMC5266282
Article Number: 28122016
Keywords: Adult;Female;*Gene Expression;Glutamate Decarboxylase/*genetics;Humans;Male;Psychotic Disorders/diagnosis/*genetics;Young Adult
Abstract: gamma-Aminobutyric acid (GABA), the primary inhibitory neurotransmitter, has often been studied in relation to its role in the pathophysiology of schizophrenia. GABA is synthesized from glutamate by glutamic acid decarboxylase (GAD), derived from two genes, GAD1 and GAD2. GAD1 is expressed as both GAD67 and GAD25 mRNA transcripts with the former reported to have a lower expression level in schizophrenia compared to healthy controls and latter was reported to be predominantly expressed fetally, suggesting a role in developmental process. In this study, GAD67 and GAD25 mRNA levels were measured by quantitative PCR (qPCR) in peripheral blood of subjects with first-episode psychosis (FEP) and from healthy controls. We observed low GAD25 and GAD67 gene expression levels in human peripheral blood. There was no difference in GAD25 and GAD67 gene expression level, and GAD25/GAD67 ratio between patients with FEP and healthy controls. PANSS negative symptoms were associated with levels of GAD25 mRNA transcripts in patients with FEP. While the current study provides information on GAD25 and GAD67 mRNA transcript levels in whole blood of FEP patients, further correlation and validation work between brain regions, cerebrospinal fluid and peripheral blood expression profiling are required to provide a better understanding of GAD25 and GAD67.
Notes: Yee, Jie Yin;Nurjono, Milawaty;Teo, Stephanie Ruth;Lee, Tih-Shih;Lee, Jimmy;eng;2017/01/26 06:00;PLoS One. 2017 Jan 25;12(1):e0170805. doi: 10.1371/journal.pone.0170805. eCollection 2017.
URL: https://www.ncbi.nlm.nih.gov/pubmed/28122016;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266282/pdf/pone.0170805.pdf
URI: https://open-access.imh.com.sg/handle/123456789/5093
Authors Address: Research Division, Institute of Mental Health, Singapore, Singapore.;Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore.;Neuroscience & Behavioural Disorders, Duke-NUS Medical School, Singapore, Singapore.;Department of General Psychiatry 1, Institute of Mental Health, Singapore, Singapore.
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